Target | Sequence of primer or probe (5’ - 3’) | Annealing temperature (˚C) | Target gene | Amplicon size (base pairs) | Reference |
Coxiella burnetii | Forward primer: AAAACGGATAAAAAGAGTCTGTGGTT | 60 | IS1111 transposase | 70 | [17] |
Reverse Primer-CCACACAAGCGCGATTCAT | |||||
Probe-[FAM]AAAGCACTCATTGAGCGCCGCG[MGB] | |||||
Borrelia burgdorferi | Forward primer CGAGTCTTAAAAGGGCGATTTAGT | 60 | 23S rRNA | 75 | [12] |
Reverse Primer-GCTTCAGCCTGGCCATAAATAG | |||||
Probe-[NED]AGATGTGGTAGACCCGAAGCCGAGTG[MGB] | |||||
Anaplasma phagocytophilum | Forward primer: ATGGAAGGTAGTGTTGGTTATGGTATT | 60 | MSP2 | 77 | [12] |
Reverse Primer: TTGGTCTTGAAGCGCTCGTA | |||||
Probe: [JOE]TGGTGCCAGGGTTGAGCTTGAGATTG[BHQ-1] | |||||
Ehrlichia Chaffeensis | Forward primer-GCGGCAAGCCTAACACATG | 60 | 16S rRNA | 81 | [18] |
Reverse Primer -CCCGTCTGCCACTAACAATTATT | |||||
Probe-[CY-5]AGTCGAACGGACAATTGCTTATAACCTTTTGGT [BBQ650] | |||||
Bartonella spp. | Fwd primer-TGCTTCGACATCCACTGTACGTC | 60 | Citrate synthase | 200 | [22] |
Rev Primer-CACCTGCTGCAATACATGCAAATG | |||||
Probe-[CY-3]TTGCAGGTTCATCAGGTGCTAATC[BHQ-2] | |||||
Rickettsia spp. | Fwd primer-ATGAATAAACAAGGKACNGGHACAC | 60 | 17 KDa antigen | 96 | [19] |
Rev Primer-AAGTAATGCRCCTACACCTACTC | |||||
Probe-[FAM]CCGAATTGAGAACCAAGTAATGC[BHQ-1] | |||||
Babesia spp. | Forward primer-CAGCTTGACGGTAGGGTATTGG | 56 | 18S rRNA | 62 | [20] |
Reverse Primer AGATGTGGTAGACCCGAAGCCGAGTG | |||||
Probe-[CY5]CGAGGCAGCAACGG[BBQ] | |||||
Borrelia spp. | Forward primer-GCTGAAGAGCTTGGAATGCAAC | 56 | Flagellin | 110 | [21] |
Reverse Primer-GCTTCATCCTGATTTGCACCAAC | |||||
Probe-[JOE]CGCGATACACCAGCATCATTATCTGAATCACAATCGCG [BHQ-1] |