Target

Sequence of primer or probe (5’ - 3’)

Annealing temperature (˚C)

Target gene

Amplicon size (base pairs)

Reference

Coxiella burnetii

Forward primer: AAAACGGATAAAAAGAGTCTGTGGTT

60

IS1111 transposase

70

[17]

Reverse Primer-CCACACAAGCGCGATTCAT

Probe-[FAM]AAAGCACTCATTGAGCGCCGCG[MGB]

Borrelia burgdorferi

Forward primer CGAGTCTTAAAAGGGCGATTTAGT

60

23S rRNA

75

[12]

Reverse Primer-GCTTCAGCCTGGCCATAAATAG

Probe-[NED]AGATGTGGTAGACCCGAAGCCGAGTG[MGB]

Anaplasma phagocytophilum

Forward primer: ATGGAAGGTAGTGTTGGTTATGGTATT

60

MSP2

77

[12]

Reverse Primer: TTGGTCTTGAAGCGCTCGTA

Probe: [JOE]TGGTGCCAGGGTTGAGCTTGAGATTG[BHQ-1]

Ehrlichia Chaffeensis

Forward primer-GCGGCAAGCCTAACACATG

60

16S rRNA

81

[18]

Reverse Primer -CCCGTCTGCCACTAACAATTATT

Probe-[CY-5]AGTCGAACGGACAATTGCTTATAACCTTTTGGT [BBQ650]

Bartonella spp.

Fwd primer-TGCTTCGACATCCACTGTACGTC

60

Citrate synthase

200

[22]

Rev Primer-CACCTGCTGCAATACATGCAAATG

Probe-[CY-3]TTGCAGGTTCATCAGGTGCTAATC[BHQ-2]

Rickettsia spp.

Fwd primer-ATGAATAAACAAGGKACNGGHACAC

60

17 KDa antigen

96

[19]

Rev Primer-AAGTAATGCRCCTACACCTACTC

Probe-[FAM]CCGAATTGAGAACCAAGTAATGC[BHQ-1]

Babesia spp.

Forward primer-CAGCTTGACGGTAGGGTATTGG

56

18S rRNA

62

[20]

Reverse Primer AGATGTGGTAGACCCGAAGCCGAGTG

Probe-[CY5]CGAGGCAGCAACGG[BBQ]

Borrelia spp.

Forward primer-GCTGAAGAGCTTGGAATGCAAC

56

Flagellin

110

[21]

Reverse Primer-GCTTCATCCTGATTTGCACCAAC

Probe-[JOE]CGCGATACACCAGCATCATTATCTGAATCACAATCGCG [BHQ-1]