| Gene | ||
CEA | Mammaglobin | 18s rRNA | |
F initiator | ACCCTGGATGTCCTCTATGG | TGCCATAGATGAATTGAAGGAATG | GTAACCCGTTGAACCCCATT |
Primer length | 20 | 24 | 20 |
R initiator | CAGGCATAGGTCCCGTTATTA | TGTCATATATTAATTGCATAAACACCTCA | CCATCCAATCGGTAGTAGCG |
Primer length | 21 | 29 | 20 |
Length of amplified segment | 174 | 89 | 152 |
Optimal annealing temperature | 61.2˚C | 61.4˚C | 53.5˚C |