Gene

CEA

Mammaglobin

18s rRNA

F initiator

ACCCTGGATGTCCTCTATGG

TGCCATAGATGAATTGAAGGAATG

GTAACCCGTTGAACCCCATT

Primer length

20

24

20

R initiator

CAGGCATAGGTCCCGTTATTA

TGTCATATATTAATTGCATAAACACCTCA

CCATCCAATCGGTAGTAGCG

Primer length

21

29

20

Length of amplified segment

174

89

152

Optimal annealing temperature

61.2˚C

61.4˚C

53.5˚C