Trefoil domain	Trefoil (P-type) domain	Reverse	AGAGCTTAGAGGGGCCACAG	[8]
18S RNA	18S ribosomal RNA gene	Forward	CCATAAACGATGCCGACTGG	[21]
18S RNA	18S ribosomal RNA gene	Reverse	TGAGGTTCCCCGTGTTGAGT	[21]
GAPDH	Glyceraldehyde 3-phosphate dehydrogenase	Forward	CTGGCATTGCTCTGAATGAC	[21]
GAPDH	Glyceraldehyde 3-phosphate dehydrogenase	Reverse	TCCACGACTCTGTTGCTGTAA	[21]
EF1A	Elongation factor 1-alpha (EF1A)	Forward	TTCGCTCCTGTCAACATCAC	[21]
EF1A	Elongation factor 1-alpha (EF1A)	Reverse	ACGTTGTCTCCAGGATTTGC	[21]
AMH	Anti-mullerian hormone	Forward	GTCCCCAGAGCGGATTCAAA	[21]
AMH	Anti-mullerian hormone	Reverse	GGTTCCCTTTCTCCGGCATT	[21]
AR	Androgen receptor (AR)	Forward	TACAGGTGGCGAGTTCCCTA	[21]
AR	Androgen receptor (AR)	Reverse	GCACCCCATTCGTTGTTCTG	[21]
CYP19	Cytochrome P450 aromatase (CYP19)	Forward	AATGTGTGCTGGAGATGCTG	[21]
CYP19	Cytochrome P450 aromatase (CYP19)	Reverse	CCAGCAGTTTCCTCTCAACC	[21]
DMRT1	Doublesex and mab-3 related transcription factor 1 (dmrt1)	Forward	GAAGGCACTGGAAACAGCTC	[21]
DMRT1	Doublesex and mab-3 related transcription factor 1 (dmrt1)	Reverse	GCAGATTGGTACGGCTCTCT	[21]
ERΑ	ESR1a mrna for estrogen receptor alpha1	Forward	CAGGCCAAGTATGGAAGGCA	[21]
ERΑ	ESR1a mrna for estrogen receptor alpha1	Reverse	CACCGCACAGAACCTCATCT	[21]
ERΒ	Estrogen receptor beta	Forward	TATCTGCCCCGCTACAAACC	[21]
ERΒ	Estrogen receptor beta	Reverse	CGCTCTCTTCTTGTACCGCA	[21]
FOXL2	Forkhead box L2	Forward	AGGCAGTGTAAGTGACGAGA	[21]
FOXL2	Forkhead box L2	Reverse	ATCCTAAACAGTCGGGGCAA	[21]
SOX9	Transcription factor SOX-9	Forward	CAGCAAAACACTCGGGAAA	[21]
SOX9	Transcription factor SOX-9	Reverse	TGGTACTTGTAATCGGGGTGA	[21]
STAR	Steroidogenic acute regulatory protein	Forward	TCGGAAACGAGAAGACAACA	[21]
STAR	Steroidogenic acute regulatory protein	Reverse	GACCAGCAAACCTCTTCAGC	[21]
VTG	Vitellogenin	Forward	CAAGTCAGCTAACCCAGCCA	[21]
VTG	Vitellogenin	Reverse	GCATGTTCAGGATCCCCCTC	[21]