Primer name

Forward (F) and (R) sequences

AT ˚C

%GC

Length (bp)

Accession number

SCARII

F: TGGATGGATGGATTGATGGA

R: AGCCAGCGAACCAAGATCTAT

60

45

20

XR269836.4

PRLI-MS04

F: GTTAGCCCCCTCCTCACTCT

R: ACCTTGCTCGTCACACCTG

60

60

20

X92380

UNH104 (G12257)

F: GCAGTTATTTGTGGTCACTA

R:GGTATATGTCTAACTGAAATCC

55

40

20

G12257

G12373 (UNH222)

F: CTCTAGCACACGTGCAT

R: TAACAGGTGGGAACTCA

53

52.94

17

G12373

G12330 (UNH136)

F: GTCACACCTCCATCATC

R: AGTTGTTTGGTCGTGTAAG

53

52.94

17

G12330

G12362 (UNH211)

F: GGGAGGTGCTAGTCATA

R: CAAGGAAAACAATGGTGATA

53

52.94

17

G12362