Expression host

GGT source

Type

pH

Temp (˚C)

Substrate Ratio of donor (M): accepter (M)

Yield (%)

Ref.

B. subtilis SK11.004

Wild type

Free enzyme

10

37

0.02:0.05

94

[30]

B. subtilis 168

B. pumilus ML413

Batch system

10

30

0.58:5.8

63

[27]

C. glutamicum SYPA5-5

B. subtilis

Batch system

10

37

0.02:0.06

86.9

[32]

E. coli K-12

Wild type

Free enzyme

10

37

0.2:1.5

60

[11]

E. coli

Wild type

Immobilized GGT cell

10

50

0.3:3

87.2

[28]

E. coli

Wild type

Free enzyme

9

37

0.048:1.6

63.8

[33]

E. coli BL21

B. subtilis 168

Free enzyme

10

37

0.2:2.2

78

[34]

E. coli BL21

B. licheniformis ER-15

Immobilized enzyme

10

37

0.08:0.6

87

[13]

E. coli BL21

E. coli K-12

Free enzyme

10.5

37

0.267:2

80

[34]

E. coli M15

E. coli NovaBlue

Free enzyme

10

37

0.01:0.04

45

[12]

E. coli M15

E. coli NovaBlue

Immobilized enzyme

10

40

0.025:0.04

27

[29]

E. coli BL21

P. nitroreducens DSM 14399

Free enzyme

10

37

0.3:1.5

40

[35]

P. nitroreducens IFO12694

Wild type

Free enzyme

11

30

0.7:1.5

39

[31]

P. nitroreducens IFO12694

Wild Type

Immobilized cell

10

40

0.025:0.25

1.43

This study

E. coli RGB

P. nitroreducens IFO12694

Immobilized cell

10

40

0.025:0.25

1.43

This study

E. coli RGB

W525D PnGGT recombinant

Immobilized cell

10

40

0.025:0.25

90

This study