Strains

Specific activity of cell-free extract

Theanine production (mM)

Hydrolysis (U_H/mg)

Transfer activity (U_T/mg)

U_T/U_H

Cell-free extract

Free cells

Immobilized cells

P. nitroreducens IFO12694

0.28

0.06

0.21

0.29

0.27

0.29

E. coli-PnGGT

13.9

3.02

0.22

0.29

0.30

0.29

E. coli-W525D

0.42

9.05

21.42

20.6

19.8

20.3

E. coli-pET22b

0.01

<0.01

0.08

0.07

0.09

0.08