Name

Expressed 3 hr post-IPTG induction?^a

Protein soluble?^b

Protein ~90% pure after phenyl sepharose purification?^b

Concentration of final fusion protein after lyophilization (μM)^c

Successful cleavage (~90%) by TEV?^d

hPin1 WW

Yes

Yes

Yes

50

Yes

SPA-WW1

Yes

Yes

Could not purify

N/A

N/A

SPANS-WW1

Yes (4 hr)

Yes

Yes^e

70 - 100

Yes (3 mL)

SPANS-WW2

Yes

Yes

Yes (plus basal cleavage in TEV)

N/A

Yes

SPANS-WW3

Yes

Yes

Yes

N/A

Yes (3 days)

K8P

Yes

Yes

Yes

N/A

Yes (10 mL)

N17F

Yes

Yes

Yes

N/A

Yes

W29A

Yes

Yes

Yes

N/A

Yes