| Vials used | SR1 | SR2 | SR3 | SR4 | SR5 | SR6 |
| Concentration of Stock Solution SM (µg∙mL−1) | 100 | 100 | 100 | 100 | 100 | 100 |
| Volume of SM collected (µL) | 400 | 800 | 1200 | 1600 | 2000 | 2400 |
| Volume of HCI used to adjust the via (mL) | 19.60 | 19.20 | 18.80 | 18.40 | 18.00 | 17.60 |
| Volume of flask used for dilution (mL) | 20 | 20 | 20 | 20 | 20 | 20 |
| Concentration of daughter solutions obtained (µg∙mL−1) | 2 | 4 | 6 | 8 | 10 | 12 |