| Sr. # | Bovine Serum Albumin (µg/µl) | Distilled Water (µl) | Bradford Reagent (µl) | O.D (595 nm) |
| 1 | 0 | 800 | 200 | 0 |
| 2 | 20 | 780 | 200 | 0.041 |
| 3 | 40 | 760 | 200 | 0.118 |
| 4 | 60 | 740 | 200 | 0.158 |
| 5 | 80 | 720 | 200 | 0.256 |
| 6 | 100 | 700 | 200 | 0.298 |
| 7 | 120 | 680 | 200 | 0.368 |
| 8 | 140 | 660 | 200 | 0.485 |
| 9 | 160 | 640 | 200 | 0.595 |
| 10 | 180 | 620 | 200 | 0.723 |
| 11 | 200 | 600 | 200 | 0.828 |