Method

Sitting-drop vapour diffusion

Plate type

48-well plates

Temperature (˚C)

18

Protein concentration (mg∙ml−1)

10

Buffer composition of protein solution

20 mM Tris-HCl at pH 8.0, 150 mM NaCl

Composition of reservoir solution

1.6 M sodium citrate tribasic dehydrate at pH 6.5.

Volume and ratio of drop

1.0 μl, 1:1 ratio of protein: reservoir solution

Volume of reservoir

70 μl