| Molecular marker technique | Primer Name | Primer sequence (5´→ 3´) | Molecular size range (bp) | TAB | PB | P (%) | TSM | |
| No. | Genotype | |||||||
| Single-SCoT | SCoT-7 | ACAATGGCTACCACTGAC | 275-1346 | 9 | 5 | 55.56 | 3 | Giza 123 (1) Giza 132 (2) |
| SCoT-8 | ACAATGGCTACCACTGAG | 233-1279 | 10 | 5 | 50.00 | 1 | Giza 132 | |
| SCoT-9 | ACAATGGCTACCACTGCC | 164-2350 | 19 | 18 | 94.74 | 4 | Giza 132 (1) Line-3 (1) Line-7 (2) | |
| Total |
|
|
| 38 | 28 | 73.68 | 8 |
|
| Double-SCoT | SCoT-7 + SCoT-8 | ACAATGGCTACCACTGAC ACAATGGCTACCACTGAG | 223-787 | 9 | 9 | 100.00 | 6 | Giza 132 (5) Line-7 (1) |
| SCoT-7 + SCoT-9 | ACAATGGCTACCACTGAC ACAATGGCTACCACTGCC | 259-3004 | 20 | 20 | 100.00 | 6 | Line-2 (2) Line-5 (1) Line-6 (1) Line-7 (2) | |
| SCoT-8 + SCoT-9 | ACAATGGCTACCACTGAG ACAATGGCTACCACTGCC | 159-720 | 9 | 5 | 55.56 | 1 | Line-4 | |
| Total |
|
|
| 38 | 34 | 89.47 | 13 |
|
| Triple-SCoT | SCoT-7 + SCoT-8 + SCoT-9 | ACAATGGCTACCACTGAC ACAATGGCTACCACTGAG ACAATGGCTACCACTGCC | 431-3043 | 18 | 17 | 94.44 | 8 | Line-2 (3) Line-5 (1) Line-8 (4) |
| Grand total |
|
|
| 94 | 79 |
| 29 |
|