| Gene/region | Oligo name | Oligo sequence | TM | Amplicon size (bp) |
| cry1Ac 3’ end | Cry1Ac 3’FP | 5’GAGTTCAACAACATCATC GC 3’ | 54.4˚C | 400bp |
| Cry1Ac 3’RP | 5’CTATTCAGCCTCGAGTGTTG 3’ | |||
| cry1Ac 5’ end | Cry1Ac 5’FP | 5’ATGGACAACAACCCAAACATCA 3’ | 59˚C | 467bp |
| Cry1Ac 5’RP | 5’ATTGAATACGCATTTCCTCGC 3’ | |||
| cry1Ac | Cry1Ac full FP | 5’ATGGACAACAACCCAAACATCAAC3’ | 60˚C | 1848bp |
| Cry1Ac full RP | 5’CTATTCAGCCTCGAGTGTTGCAGTA3’ | |||
| 35Sde promoter (only for DNA-PCR) | TPchk-Cry1Ac FP | 5’GCTTGCATGCCTGCAGGT 3’ | 60˚C | 847bp |
| TPchk-Cry1Ac RP | 5’CCCCAGATGATGTCAACTAGTCCG3’ | |||
| nptII | NptII FP | 5’TTTGTCAAGACCGACCTGTC 3’ | 58˚C | 650bp |
| NptII RP | 5’AAGAACTCGTCAAGAAGGCG 3’ | |||
| Ubiquitin-conjugating enzyme (UCE) | UCE FP | 5’GAGGATTCTTTCTGAGGGGTTT 3’ | 55˚C | 230bp |
| UCE RP | 5’AGGGAAGTGAATGGTAACGAGA 3’ |