Gene Name - Method

Forward Primer

Reverse Primer

axin2 - qRT-PCR

CCTTACATTCGCAGCCGGAGTCTG

TGAGAGCGATCTGTACTGTCAGCTG

dlx2a - qRT-PCR

GCACCTACGGCTCCTGCTCCTC

CAGGGCCGCCAGCTGGAAAC

edn1 - qRT-PCR

GCAAACACACGCTGGCAGAAACA

GAAATCCACGCTTGGCTTCTGTG

grem2 - qRT-PCR

GCACACACTCCAGCAGGATGAG

GGAGGCGAGCACTTCTTGTTTGC

jag1b - qRT-PCR

GGCACTGGTACCTGCTGAAGAAC

CCTGGGCTCCTCTGTGGAAATG

msxe - qRT-PCR

CGCCTGGCGTTTTCGGTGGA

CCGCGGCTTCCGGTTGGTTT

tbx22-2 - qRT-PCR

cacgcgacaagtggatcata

CACTAACCCTGTGCGTCAAA

Wnt3a - qRT-PCR

GGCATCCAGGAGTGTCAGCATC

CCGAGGCTATAGCATGCACGAAC

Wnt5b - qRT-PCR

CAGACAGAGGCGATGGAACTGCAG

CGCTCGACTCACAGCATTCACAAC

Wnt9a - qRT-PCR

CTCCGTGCCCGGATAGACATGC

GCACGGTGCATGAGCCCGAAAC

dlx1a - WISH

CCGCAAGCCCCGGACCATATACTC

CTCCGGTGCGACCGGACGAC

dlx2a - WISH

CCTCACCAACTCCTGCAGATG

ggcaatgatcaacgtggcatc

edn1 - WISH

CTACTACTGGAATACGGGACTTG

CATTTCATAGAAATATCACTCATTAGCC

Frzb - WISH

GAGTGAAGGAGATCAGGATTAGAAATC

GCCACCCATTCCTAACACAGC

jag1b - WISH

CTGCATTGCTAATGGACAAGTAAC

CACAGGGCAAATAACAGCCTTGGAGTCC

pitx2 - WISH

CGAAGATTCGAACGATGACC

TGGGATGTTGAAAAACGAAA

sox9a - WISH

GACGTCAATGAGTTTGACCAATACCTC

gggcatgcaaattaagtagaacattac

Wnt3a - WISH

ATGATATATCTTGGATATTTCC

CACATGTGTCTAATCTGGTTTTGG

Wnt5b - WISH

GGACAACGTCAACTATGGCTAC

cacgtctgCTACTTGCACAC