| Category | Proposed method | Reported method [9] | Reported method [10] |
| Reagents/Instruments | Penalty points | ||
| Methanol | 12 | 12 | 12 |
| Water | 0 |
|
|
| Phosphate buffer |
|
| 2 |
| Acetonitrile |
|
| 4 |
| Sodium hydroxide |
|
| 2 |
| Formic acid |
| 12 |
|
| Diethyl ether |
| 6 |
|
| Ethanol | 3 |
|
|
| Centrifuge | 1 | 1 | 1 |
| Sonicator | 1 |
| 1 |
| HPLC |
| 2 | 2 |
| UPLC | 0 | 0 | 0 |
| Occupational hazard | 0 | 0 | 0 |
| Waste | 5 | 5 | 5 |
| Total penalty points | 22 | 38 | 29 |
| Analytical Eco-Scale total | 100 − 22 = 79 | 100 − 38 = 62 | 100 − 29 = 71 |
|
| Excellent | Acceptable | Acceptable |