FEB

UHPLC method

Reported method ^[10]

Taken (µg·mL⁻¹)

Found* (μg/mL)

% Recovery

Taken (μg/mL)

Found * (μg/mL)

% Recovery

0.4

0.395

98.75

5

5.06

101.19

0.8

0.813

101.63

10

10.136

101.36

1.2

1.194

99.5

15

14.822

98.81

1.6

1.605

100.31

20

19.812

99.06

2

2.024

101.2

25

25.273

101.09

Mean % ± SD

100.28% ± 1.185

100.30% ± 1.255

t-test

0.031 (2.306)

-

F-test

1.121 (6.388)

-

DIC

1

1.006

100.6

20

19.99

99.95

2

1.988

99.4

30

29.817

99.39

3

3.041

101.37

40

40.268

100.67

4

3.975

99.38

50

50.105

100.21

5

4.942

98.84

60

59.898

99.83

Mean % ± SD

99.92% ± 1.036

100.01% ± 0.473

t-test

0.181 (2.306)

-

F-test

4.792 (6.388)

-