Tailing factor

Resolution

No. of theoretical plates

Parameter

DIC

FEB

DIC

FEB

1.18

-

-

4244

-

Intersil C₁₈

Column

1.22

1.32

3.41

3916

3608

BDS hypersil C₁₈ column

1.11

1.09

5.18

4787

4558

phenomenex kinetex C₁₈ column

1.73

1.24

5.99

3112

4215

ACN-buffer

Mobile phase

1.11

1.09

5.18

4787

4558

Ethanol-water

---

1.16

---

---

4459

Methanol-buffer

1.97

2.05

4.61

572

485

5%

Ethanol

concentration (%v/v)

1.23

1.27

4.12

1556

2300

10%

1.11

1.09

5.18

4787

4558

15%

1.53

1.85

3.83

3646

2859

25%

1.81

2.04

3.29

2957

3152

30%

1.26

1.38

3.91

2804

2277

0.6

Flow rate

(mL/min)

1.14

1.26

5.26

3915

3887

0.8

1.11

1.09

5.18

4787

4558

1.0

1.16

1.18

4.83

2961

2486

1.2