reagent (enzyme) | final concentration (colorimetric) |
5 mmol/L phosphate buffer (pH 7.8) |
|
130 mmo/L methionine (MET) solution | 13 mmo/L |
750 μmol/L nitroblue tetrazolium (NBT) solution | 75 μmol/L |
100 μmol/LNa2-EDTA solution | 10 μmol/L |
20 μmol/L riboflavin solution | 2 μmol/L |
enzyme solution | control branch is replaced by buffer |
total capacity | 3.0 |