reagent (enzyme)

final concentration (colorimetric)

5 mmol/L phosphate buffer (pH 7.8)

130 mmo/L methionine (MET) solution

13 mmo/L

750 μmol/L nitroblue tetrazolium (NBT) solution

75 μmol/L

100 μmol/LNa2-EDTA solution

10 μmol/L

20 μmol/L riboflavin solution

2 μmol/L

enzyme solution

control branch is replaced by buffer

total capacity

3.0