| Run | X1 (˚C) temperature | X2(rpm) Oscillator speed | X2 (%) inoculum concentration | X4 pH | degradation Rate (%) |
| 1 | 0 (35) | 0 (120) | −1 (4) | −1 (6) | 79.89 |
| 2 | 0 | 0 | 1 (8) | 1 (8) | 85.70 |
| 3 | 0 | 1 (160) | 0 (6) | −1 | 84.26 |
| 4 | 1 (40) | 1 | 0 | 0 (7) | 89.89 |
| 5 | 0 | −1 (80) | 0 | 1 | 83.31 |
| 6 | 1 | 0 | 1 | 0 | 89.69 |
| 7 | −1 (30) | 0 | −1 | 0 | 79.59 |
| 8 | −1 | 0 | 0 | −1 | 81.99 |
| 9 | 0 | 0 | 0 | 0 | 94.09 |
| 10 | 0 | 0 | 0 | 0 | 95.80 |
| 11 | −1 | −1 | 0 | 0 | 78.39 |
| 12 | 0 | −1 | 0 | −1 | 81.78 |
| 13 | 1 | 0 | −1 | 0 | 89.85 |
| 14 | −1 | 1 | 0 | 0 | 75.56 |
| 15 | 0 | 1 | 0 | 1 | 93.45 |
| 16 | 0 | 1 | 1 | 0 | 90.53 |
| 17 | 1 | 0 | 0 | 1 | 77.88 |
| 18 | 0 | 0 | 0 | 0 | 95.39 |
| 19 | 0 | 0 | 0 | 0 | 96.60 |
| 20 | 1 | −1 | 0 | 0 | 82.09 |
| 21 | 0 | −1 | 1 | 0 | 82.60 |
| 22 | 0 | 0 | 1 | −1 | 92.51 |
| 23 | 0 | 0 | −1 | 1 | 84.96 |
| 24 | 0 | 0 | 0 | 0 | 95.72 |
| 25 | −1 | 0 | 1 | 0 | 86.63 |
| 26 | 1 | 0 | 0 | 1 | 88.15 |
| 27 | 0 | 1 | −1 | 0 | 82.19 |
| 28 | 1 | 0 | 0 | −1 | 89.03 |
| 29 | 0 | −1 | −1 | 0 | 84.44 |