Standard run

Composition parameters

Experimental observed

Predictions

PLGA (%w/v)

Cip-HCl

(%w/v)

PVA

(%w/v)

Size

(nm)

DR

(%w/w)

DL

(%w/w)

Size

(nm)

DR

(%w/w)

1

1.0

0.5

0.1

443

5.85

1.125

443.00

5.85

2

1.0

1.0

0.3

235

3.70

1.490

237.33

4.89

3

1.0

1.5

0.2

302

1.70

0.990

299.67

0.51

4

1.5

0.5

0.3

372

15.70

1.455

369.67

14.51

5

1.5

1.0

0.2

384

4.28

1.425

384.00

4.28

6

1.5

1.5

0.1

436

2.96

1.155

438.33

4.15

7

2.0

0.5

0.2

347

8.86

1.055

349.33

10.05

8

2.0

1.0

0.1

358

5.28

1.055

355.67

4.09

9

2.0

1.5

0.3

353

3.21

1.120

353.00

3.21

Validation and optimization experiments

1 V

1

0.2

0.1

530

7.83

1.618

558.84

10.01

2 V

1

0.5

0.4

202

8.49

1.301

215.00

19.38