| Test | P. aeruginosa | S. aureus | E. coli |
| Indole | + | − | + |
| Methyl red | − | − | − |
| Voges-Proskauer | − | + | − |
| Gelatine | − | + | − |
| Catalase | − | + | + |
| Oxidase | + | − | + |
| Nitrate | + | + | + |
| Starch | − | − | − |
| Citrate | − | − | − |