| Reagent | Volume |
| Sample (peripheral blood) | 100 μl |
| RPMI (HEPES and glutamine) | 5 ml |
| Fetal Bovine Serum | 500 μl |
| Phytohemagglutinin | 10 μl |
| Streptopenicillin | 100 μl |
| Colchicine | 100 μl |
| Hypotonic solution at 0.56% | |
| Total incubation time of 60 hs | |
| Incubation temperature 28˚C | |