Reagent | Volume |
Sample (peripheral blood) | 100 μl |
RPMI (HEPES and glutamine) | 5 ml |
Fetal Bovine Serum | 500 μl |
Phytohemagglutinin | 10 μl |
Streptopenicillin | 100 μl |
Colchicine | 100 μl |
Hypotonic solution at 0.56% | |
Total incubation time of 60 hs | |
Incubation temperature 28˚C |