| Factors | Unit | Notation | Range and levels | ||
| −1 | 0 | 1 | |||
| Ultrasonic power | W | X1 | 150 | 200 | 250 |
| Ultrasonication time | min | X2 | 10 | 15 | 20 |
| Extraction temperature | ˚C | X3 | 40 | 50 | 60 |