SNP | Primer sequence (5’ to 3’) | Primer conc. | Melting Temp | Annealing Temp. | Expected products |
| Forward outer primer GTCATAGGTCAAACAGCTAGATATTGGGT | 0.04 µM | 63˚C |
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rs972283 | Reverse outer primer TCTACAGGACCAACTCAAATTATGAGGT | 0.04 µM | 63˚C | 60˚C | Common 437 bp |
(A/G) | Forward inner primer (G allele) TCATTGTATACTTGGAAAAAATCCTACATG | 0.16 µM | 62˚C |
| G allele 221 bp |
| Reverse inner primer (A allele) TATGTAAAAATAAGTATGCGCCATGCCT | 0.04 µM | 65˚C |
| A allele 274 bp |