| Parameters | Conditions |
| System | Continuously |
| Salinity | 32 ± 0.29 |
| Temperature | 20 ± 1.75 |
| pH | 7.74 ± 0.16 |
| Dissolved Oxygen (%) | 84.14 ± 6.33 |
| Photoperiod | 16:8 (Artificial light) |
| Type of Aquarian | Glass, transparent, with cover |
| Type of Water | Artificial seawater (MiliQ® with marine salt) |
| Water Volume | 2.5 L (each Aquarian) |
| Water change | Continuously |
| Aeration | Continuously |
| N˚ of Actinia equina (adult) | 100 |
| N˚ of Actinia equina | 100 |
| Number of Replicates (each Aquarian) | 2 |
| Diet | Each 3 days* |
| Monitoring | Daily (each 6 hours) |
| *Feed diet fish |
|