Genetic

polymorphism

Primer sequence

Annealing

temperature

Amplicon size

rs4731702 (C/T)

Forward inner primer (C allele):

5’AAAAAACAGCATTATTTCCCACACAAAC 3’

Reverse inner primer (T allele):

5’ TATCTTTTTGGTGCTAAATGGAACGGA 3’

Forward outer:

5’ CCCAAGGCATCTATCCAAAA 3’

Reverse Outer:

5’ CCGTTGAACTGTGTTTGCAC 3’

62˚C

515 bp (C allele)

58 bp (T allele)

619 bp

(from two outer primers)