Genetic polymorphism | Primer sequence | Annealing temperature | Amplicon size |
rs4731702 (C/T) | Forward inner primer (C allele): 5’AAAAAACAGCATTATTTCCCACACAAAC 3’
Reverse inner primer (T allele): 5’ TATCTTTTTGGTGCTAAATGGAACGGA 3’
Forward outer: 5’ CCCAAGGCATCTATCCAAAA 3’
Reverse Outer: 5’ CCGTTGAACTGTGTTTGCAC 3’ | 62˚C | 515 bp (C allele)
58 bp (T allele)
619 bp (from two outer primers)
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