Genetic polymorphism | Primer sequence | Annealing temperature | Amplicon size |
rs4731702 (C/T) | Forward inner primer (C allele): 5’AAAAAACAGCATTATTTCCCACACAAAC3’ | 62˚C | 515 pb (C allele) |
Reverse inner primer (T allele): 5’TATCTTTTTGGTGCTAAATGGAACGGA3’ |
| 158 bp (T allele) | |
Forward outer: 5’CCCAAGGCATCTATCCAAAA3’ |
| 619 bp (from two outer primers) | |
Reverse Outer: 5’CCGTTGAACTGTGTTTGCAC3’ |