Genetic

polymorphism

Primer sequence

Annealing

temperature

Amplicon size

rs4731702 (C/T)

Forward inner primer (C allele):

5’AAAAAACAGCATTATTTCCCACACAAAC3’

62˚C

515 pb (C allele)

Reverse inner primer (T allele):

5’TATCTTTTTGGTGCTAAATGGAACGGA3’

158 bp (T allele)

Forward outer:

5’CCCAAGGCATCTATCCAAAA3’

619 bp

(from two outer primers)

Reverse Outer:

5’CCGTTGAACTGTGTTTGCAC3’