| Sample | Volume | |||
| BSA (25 mg/mL) | ddH2O | Biuret A | Biuret B | |
| Standard sample 1 | 0 | 1 | 0.5 | 0.5 |
| Standard sample 2 | 0.2 | 0.8 | 0.5 | 0.5 |
| Standard sample 3 | 0.4 | 0.6 | 0.5 | 0.5 |
| Standard sample 4 | 0.6 | 0.4 | 0.5 | 0.5 |
| Standard sample 5 | 0.8 | 0.2 | 0.5 | 0.5 |
| Standard sample 6 | 1 | 0 | 0.5 | 0.5 |