Experiment

No

A

Temperature

(˚C)

B

inoculation time (h)

C

liquid volume (ml)

γ-PGA yield (g/L)

1

1

1

1

46.51

2

2

1

2

39.45

3

3

1

3

40.73

4

1

2

2

34.35

5

2

2

3

38.85

6

3

2

1

124.58

7

1

3

3

26.82

8

2

3

1

82.21

9

3

3

2

77.81

k1

107.68

126.69

253.3

k2

160.51

197.78

151.61

k3

243.12

186.84

106.4

$\overline{k}1$

35.89

42.23

84.43

$\overline{k}2$

53.50

65.93

50.54

$\overline{k}3$

81.04

62.28

35.47

R range

45.15

23.7

48.96