No: of samples: 12

Reagents

Volume for 1 rxn (µl)

Vol. for master

+ 10% (µl)

Vol. for master + 1 rxn (µl)

Vol. for master + 2 rxn (µl)

2 × Quantitect master-mix

10

121

120

130

Forward primer (20 µl)

0.2

2.42

2.4

2.6

Reverse primer (20 µl)

7.4

89.54

88.8

96.2

Total

17.8

215.38

213.6

231.4

Template DNA (per rxn): 2 µl