No: of samples: 12 | ||||
Reagents | Volume for 1 rxn (µl) | Vol. for master + 10% (µl) | Vol. for master + 1 rxn (µl) | Vol. for master + 2 rxn (µl) |
2 × Quantitect master-mix | 10 | 121 | 120 | 130 |
Forward primer (20 µl) | 0.2 | 2.42 | 2.4 | 2.6 |
Reverse primer (20 µl) | 7.4 | 89.54 | 88.8 | 96.2 |
Total | 17.8 | 215.38 | 213.6 | 231.4 |
Template DNA (per rxn): 2 µl |