| Runs | Fermentation time (X1)/hrs. | pH (X2)/na | Biomass load (X3)/g/mL | X1 | X2 | X3 |
| 1 | 1 | 1 | −1 | 72 | 7 | 15 |
| 2 | 0 | 0 | −1.6818 | 48 | 6 | 11.5911 |
| 3 | −1 | −1 | 1 | 24 | 5 | 25 |
| 4 | −1 | 1 | −1 | 24 | 7 | 15 |
| 5 | 1 | 1 | 1 | 72 | 7 | 25 |
| 6 | 0 | 0 | 0 | 48 | 6 | 20 |
| 7 | 0 | −1.6818 | 0 | 48 | 4.31821 | 20 |
| 8 | −1 | −1 | −1 | 24 | 5 | 15 |
| 9 | 0 | 0 | 1.68179 | 48 | 6 | 28.409 |
| 10 | 1.68179 | 0 | 0 | 88.363 | 6 | 20 |
| 11 | 1 | −1 | −1 | 72 | 5 | 15 |
| 12 | 0 | 0 | 0 | 48 | 6 | 20 |
| 13 | −1.6818 | 0 | 0 | 7.63704 | 6 | 20 |
| 14 | 0 | 1.68179 | 0 | 48 | 7.68179 | 20 |
| 15 | 1 | −1 | 1 | 72 | 5 | 25 |
| 16 | −1 | 1 | 1 | 24 | 7 | 25 |