|
| Gag and pol | Nested pol and gag |
| Denaturation initial | 95˚C: 9 min | 95˚C: 9 min |
| Denaturation per round | 94˚C: 1 min | 94˚C: 1 min |
| Hybridization Per round | 50˚C: 1 min | 50˚C: 1 min |
| Elongation Per round | 72˚C: 1 min | 72˚C: 1 min |
| Elongation Final | 72˚C: 10 min | 72˚C: 10 min |
| hold | 4˚C | 4˚C |
| number of cycle | 40 | 40 |