| Initiation denaturation | 94˚C | 5 min |
| Denaturation | 94˚C | 30 sec |
| Annealing | 58˚C | 30 sec |
| Elongation | 72˚C | 30 sec |
| Final elongation | 72˚C | 10 min |
| Refrigerator | 4˚C | Infinity |