Reference

Ethanol concentration v/v

Mixing, heating at 60˚C for 30 min, cooling and centrifuge or filtering to down Mn(IV) precipitate.

Absorbance

375 µl:100 ml

Absorbance difference

(A_blank − A_sample)

A

Ref.

ΔA

Ref.

Blank

0%

1 ml H₂O + 5 ml of 5% permanganate

2.103

A_B

-

-

S1

1%

1 ml of 1% Alcohol + 5 ml of 5% permanganate

1.812

A_S1

0.291

A_B − A_S1

S2

2%

1 ml of 2% Alcohol + 5 ml of 5% permanganate

1.502

A_S2

0.601

A_B − A_S2

S3

3%

1 ml of 3% Alcohol + 5 ml of 5% permanganate

1.211

A_S3

0.892

A_B − A_S3

S4

4%

1 ml of 4% Alcohol + 5 ml of 5% permanganate

0.934

A_S4

1.169

A_B − A_S4

S5

5%

1 ml of 5% Alcohol + 5 ml of 5% permanganate

0.631

A_S5

1.472

A_B − A_S5

S6

6%

1 ml of 6% Alcohol + 5 ml of 5% permanganate

0.363

A_S6

1.797

A_B − A_S6