Material Name | Role |
Sterile cotton stick | It was used to collect vaginal swab samples |
Normal saline | It was used for irrigation |
Sterile container | It was used for sample transportation |
Microscope | To identify Microorganisms in the collected samples |
Blood Agar (HIMEDIA® Ref M073-500G), | This is enriched media which was used to isolate fastidious organisms |
Mannitol Salt Agar (HIMEDIA® Ref M118-500G), | This a selective and differential media used to isolate staphylococcus species |
MacConkey Agar (HIMEDIA® Ref M081-500G) | It was used to isolate gram-negative enteric bacteria. It helped in differenciating lactose fermenting from lactose non-fermenting bacteria |
Sabouraud Dextrose Agar (TM Media Ref TM 387) | This is non-selective media used to isolate fungi and yeasts in our studt experiments |
Kligler’s Iron Agar (HIMEDIA® Ref M078-500G) | This media was used for the identification of Enterobacteriaceae, based on double sugar fermentation and hydrogen sulphide production |
Simon’s Citrate Agar (HIMEDIA® Ref M099-500G). | This media was used to differenciate gram-negative bacteria basing on the citrate utilization |
Urea Broth (HIMEDIA® Ref M111-500G) | This is a defferential medium that was used to test for ability of organisms to produce urease |
Sulphide Indole Motility (HIMEDIA® Ref M181-500G) | This medium was used to differentiate enteric organisms based on the ability to produce indole and hydrogen sulfide |