PCR gag and pol

PCR env and Nested pol

PCR HLA and Nested gag

Nested env

Denaturation

95˚C/9 minutes

95˚C/9 minutes

95˚C/9 minutes

95˚C/9 minutes

Denaturation

94˚C/1 minute

94˚C/1 minute

94˚C/1 minute

94˚C/1 minute

Hybridation

50˚C/1 minute

50˚C/1 minute

55˚C/1 minute

45˚C/1 minute

Elongation

72˚C/1 minute

72˚C/1 minute

72˚C/1 minute

72˚C/1 minute

Final Elongation

72˚C/10 minutes

72˚C/10 minutes

72˚C/10 minutes

72˚C/10 minutes

Hold

4˚C

4˚C

4˚C

4˚C

Number of cycles*

35

30

25

25