| PCR assay | Primers | Sequences |
| HLA assay | GH26 Forward | 5’-GTGCTGCAGGTGTAAACT-3’ |
| GH27 Reverse | 5’-CACGGATCCGGT-3’ | |
| gag | GAG1 Forward | 5’-GGTACATCAGGCCATATCACC-3’ |
| GAG4 Reverse | 5’-ACCGGTCTACATAGTCTC-3’ | |
| pol | POLITG1 Forward | 5’-CCCTACAATCCCCAAAGTCAAGG-3’ |
| POLITG4 Reverse | 5’-TACTGCCCCTTCACCTTTCCA-3’ | |
| env | ENV1 Forward | 5’-GAGGATATAATCAGTTTATGG-3’ |
| ENV4 Reverse | 5’-AATTCCATGTGTACATTGTACTG-3’ | |
| Nested gag | GAG2 Forward | 5’-GAGGAAGCTGCAGAATGGG-3’ |
| GAG3 Reverse | 5’-GGTCCTTGTCTTATGTCC-3’ | |
| Nested pol | POLITG2 Forward | 5’-TAAGACAGCAGACAAATGGCAG-3’ |
| POLITG3 Reverse | 5’-GCTGTCCCTGTAATAAACCCG-3’ | |
| Nested env | ENV2 Forward | 5’-GATCAAAGCCTAAAGCCATG-3’ |
| ENV3 Reverse | 5’-CAATAATGTATGGGAATTGG-3’ |