Day | Approximate time used | Content |
1 | 1 h | Introductory remarks on: biosafety in the handling of chemical and biological materials procedures on DNA extraction protocols |
2:30 h | DNA extraction solutions preparation and autoclaving | |
30 min | Blood sample collection | |
1:30 h | Protocol 1 starting. Hemolysis and nucleus lysis, overnight | |
1:30 h | Protocol 2 starting. Hemolysis and nucleus lysis, overnight | |
2 | 3:30 h | DNA extraction using Protocol 3 |
2 h | Protein extraction and DNA precipitation of samples from protocols 1 and 2 | |
3 | 2 h | DNA recover, wash and solubilization of samples from protocols 1 and 2 |
1:30 h | Spectrophotometric quantification of DNA extracted from protocols 1, 2 and 3 | |
2:30 h | Agarose gel electrophoresis of Genomic DNA | |
4 | 4 h | PCR reaction |
2 h | Agarose gel electrophoresis of PCR products | |
5 | 4 h | Graphics and Statistical analysis of the results to answer the questions above: I. Using spectrophotometer quantification calculate mean and standard deviation of DNA solutions: a) Concentrations b) 260/280 ratio c) Yield per protocol d) Yield per blood volume used II. Construct graphics using the calculated values for a, b, c and d items III. Identify the statistical difference among the three DNA extraction techniques on a, b, c and d items |