| Parameter | Value | Column 1 | Column 2 |
| Instrument settings | Agilent 5977B MSD conditions | ||
| Vial pressurization gas | Helium | Acquisition mode | Scan (20 m/z - 450 m/z)) |
| Loop size | 1 mL | Solvent delay | 1.73 minutes |
| Keyboard lock | ON | Tune file | etune.u |
| Transfer line type | Fused Silica | EM Setting mode | Gain = 1 |
| Transfer line diameter | 0.53 mm | Scan speed | 781 [N = 3] |
| System configuration | Scan parameters | ||
| Carrier control | GC Instrument | Threshold | 50 |
| Oven temperature | 140˚C | MSD Source temperature | 280˚C |
| Loop temperature | 140˚C | MSD Quadrupole temperature | 150˚C |
| Transfer line temperature | 160˚C | ||
| Vial equilibration | 5.00 minutes | ||
| Injection duration | 0.25 minutes | ||
| GC cycle time | 30 minutes | ||
| Vial size | 20 mL | ||
| Vial shaking | Level 2, 25 shakes/min | ||
| Fill mode | Default | ||
| Fill pressure | 15 psi | ||
| Loop fill mode | Custom | ||
| Loop ramp rate | 20 psi/min | ||
| Loop final pressure | 10 psi | ||
| Loop equilibration time | 0.05 minutes | ||
| Carrier control mode | GC controls carrier | ||
| Extraction mode | Single extraction | ||
| Vent after extraction | ON | ||
| Post injection purge | 100 mL/min for 1 minute |
| |
| Acceptable leak check | Default, 0.2 mL/min | ||
| Agilent 7890B GC conditions | |||
| GC oven temperature | 40˚C | ||
| Hold time | 5 minutes | ||
| Oven program | 15˚C/min to 160˚C, hold 0 minutes, then 35˚C/min to 300˚C, hold 7 minutes |
| |
| Equilibration time | 0.1 minute | ||
| Max temperature | 320˚C | ||
| Column flow | 1.2 mL/min | ||
| Font S/SL inlet He | Split | ||
| Column specifications | |||
| Catalog number | Restek 13868 | ||
| Product name | Rxi-624Sil MS | ||
| Size | 30 meter × 0.25 mm ID × 1.4 µm df |
| |
| Max temperature | 320˚C | ||