Constructs

Number of methionines

(type of mutations)

Independently

transformed plants

PCR-positive

plants

ELISA-phaseolin protein positive plants

pTRA 451

3 (positive control wild-type)

22

20

13

pTRA 452

18 (two loop-insertions)

18

16

0

pTRA 453

8 (negative control, Hoffman helix-insertion)

9

8

0

pTRA 454

13 (C-barrel substitution)

12

10

0

pTRA 455

23 (N- & C-barrel substitutions)

19

10

0

pTRA 456

33 (two loop-insertions and N- & C-barrel substitutions)

35

25

0