Constructs | Number of methionines (type of mutations) | Independently transformed plants | PCR-positive plants | ELISA-phaseolin protein positive plants |
pTRA 451 | 3 (positive control wild-type) | 22 | 20 | 13 |
pTRA 452 | 18 (two loop-insertions) | 18 | 16 | 0 |
pTRA 453 | 8 (negative control, Hoffman helix-insertion) | 9 | 8 | 0 |
pTRA 454 | 13 (C-barrel substitution) | 12 | 10 | 0 |
pTRA 455 | 23 (N- & C-barrel substitutions) | 19 | 10 | 0 |
pTRA 456 | 33 (two loop-insertions and N- & C-barrel substitutions) | 35 | 25 | 0 |