Program | Steps | Temperature (˚C) | Time | Cycles |
1 | Initial denaturation | 94 | 2 min (3 min) | 1 |
2(1) | Denaturation | 94 | 45 s |
|
2(2) | glmM | 56 | 30 s | 40 |
TNF-α Alignment | 60 | 20 s | 40 | |
2(3) | Extension | 72 | 1 min |
|
3 | Final extension | 72 | 7 min | 1 |
4 | Storage | 4 | Whatever time is necessary | 1 |