Program

Steps

Temperature (˚C)

Time

Cycles

1

Initial denaturation

94

2 min (3 min)

1

2(1)

Denaturation

94

45 s

2(2)

glmM

56

30 s

40

TNF-α Alignment

60

20 s

40

2(3)

Extension

72

1 min

3

Final extension

72

7 min

1

4

Storage

4

Whatever time is necessary

1