| HILIC | Ion pairing | |||||||
| Aminoglycosides | R2 | LOD (ppb) | S/N | Tr (min) | R2 | LOD (ppb) | S/N | Tr (min) |
| Amikacin | 0.991 | 10 | 3.12 | 9.7 | 0.998 | 5 | 148 | 8.2 |
| Apramycin | 0.988 | 90 | 3.96 | 5.3 | 0.991 | 5 | 138 | 8.8 |
| Dihydrostreptomycin | 0.996 | 5 | 11.4 | 5.4 | 0.994 | 5 | 57.4 | 7.2 |
| Gentamicin | 0.989 | 60 | 4.99 | 5.3 | 0.989 | 5 | 204 | 9.1 |
| Hygromycin | 0.994` | 5 | 8.08 | 7.2 | 0.949 | 5 | 10 | 7.2 |
| Kanamycin | 0.995 | 90 | 3 | 10.2 | 0.982 | 5 | 138 | 8.3 |
| Neomycin | 0.420 | 200 | 4.05 | 5.3 | 0.994 | 5 | 161 | 9.3 |
| Paromomycin | 0.981 | 200 | 3.1 | 5.1 | 0.991 | 5 | 277 | 8.8 |
| Sisomycin | 0.979 | 200 | 2.10 | 5.2 | 0.990 | 5 | 168 | 9 |
| Spectinomycin | 0.942 | 5 | 52 | 6.7 | 0.979 | 15 | 12.6 | 6.7 |
| Streptomycin | 0.987 | 5 | 9.06 | 5.4 | 0.993 | 15 | 17.6 | 9.1 |
| Tobramycin | 0.968 | 200 | 3.4 | 5.3 | 0.987 | 5 | 169 | 8.9 |