Sahoo et al., 2011

Kant et al., 2007

Cambia Protocol

Mohanty et al., 1999

Modified Method

Media

MS 4.4 g

MS 4.4 g

N 64 g

MS 4.4 g

MS 4.4 g

Proline

-

560 mg

500 mg

-

500 mg

Casein hydrolysate

300 mg

300 mg

1 gm

300 mg

Sugar

Maltose 30 g

Maltose 30 g

Sucrose 30 mg

Sucrose 30 mg

Sorbitol 30 g

Matrix

0.8% Agar

1% Agarose

0.6% Phytagel

0.4% Phytagel

1% Agarose

NAA (mg/l)

0.5

0.5

0.5

0.5

Kinetin (mg/l)

-

2

-

-

2

BAP (mg/l)

3

-

3

1

1

Glutamine

-

-

.5

-

-

Fe₂EDTA

-

-

10 ml (100´)

-

-