| Gene | Variant | Location | Genotyping Method | PCR Product (bp) | Annealing Temperature, MgCl2 concentration | Restriction enzyme | Restriction digestion pattern | Primers Reference |
| BRCA1 | p.Pro871Leu (c.2612C > T) | Exon 10 | PCR-RFLP | 125 | 59˚C, 1.5 mM | Hpa II | C allele-99 and 26 T allele-125 | [15] |
|
| p.Cys64Arg (c.190T > C) | Exon 5 | PCR-RFLP | 193 | 59˚C, 1.5 mM | SnaB I | T allele-193 C allele-124 and 69 | [16] |
|
| 130delT | Exon 11 | PCR-RFLP | 151 | 59˚C, 1.5 mM | Dde I | Wt-151 Mut-96 and 55 | [17] |
|
| p.G1738R (g.5331G > A) | Exon 20 | PCR-RFLP | 233 | 59˚C, 1.5 mM | BsaX I | G allele-112, 91, 30 A allele-233 | [18] |
| TP53 | p.P47S | Exon 4 | PCR-RFLP | 201/185* | 59˚C, 1 mM | MspI | S allele-201/185 P allele-156/140, 45 | [19] |
|
| p.R72P | Exon 4 | PCR-RFLP | 279 | 59˚C, 1 mM | BstUI | P allele 279 R allele-160 and 119 | [20] |
|
| PIN3 Ins 16 bp | Intron 3 | PCR | - | 61˚C, 1 mM |
| A1 allele-119 A2 allele-135 | [21] |
|
| p.R213R | Exon 6 | PCR-RFLP | 1621 | 59˚C, 1.5 mM | TaqI | A allele-312, 383 and 926 G allele-695 and 926 | [22]
[22]
|
|
| r.13494g > a | Intron 6 | PCR-RFLP | 1621 | 59˚C, 1.5 mM | MspI | G allele-356, 277, 277, 299, 168, 124 and 120 A allele-633, 299, 277, 168, 124, 120 | |
| VEGF | −2549I/D | Promoter | PCR | 229/211 | 59˚C, 1.5 mM | - | I allele 229 D allele 211 | [23] |
|
| −2578C/A | Promoter | PCR-RFLP | 459 | 59˚C, 1.5 mM | BglII | C allele-459 A allele-247, 212 | [24] |
|
| +936C/T | 3'UTR | PCR-RFLP | 207 | 59˚C, 1.5 mM | NlaIII | C allele-207 T allele-122,85 | [25] |
|
| −417C/T | Promoter | Sequencing | 486bp | 62˚C, 1.5 mM | - | - | [26] |
|
| −172C/A | Promoter | Sequencing |
|
| - | - |
|
|
| −165C/T | Promoter | Sequencing |
|
| - | - |
|
|
| −160C/T | Promoter | Sequencing |
|
| - | - |
|
|
| −152G/A | Promoter | Sequencing |
|
| - | - |
|
|
| −141A/C | Promoter | Sequencing |
|
| - | - |
|
|
| −116G/A | Promoter | Sequencing |
|
| - | - |
|
|
| −7C/T | 5' UTR | ARMS-PCR |
| 59˚C, 1.5 mM |
| Control-425 C and T allele-183 | [27] |
| HIF1A | g.C111A | Exon 2 | PCR-RFLP | 187 | 59˚C, 1.5 mM | BglII | C allele-44, 143 A allele-187 | [28]
[28]
[28] |
|
| g.C1772T | Exon 12 | PCR-RFLP | 346 | 55˚C, 1.5 mM | HphI | C allele 118, 228 T allele 346 | |
|
| g.G1790A | Exon 12 | PCR-RFLP | 346 | 55˚C, 1.5 mM | AciI | G allele 145, 201 A allele 346 | |
| MDM2 | SNP309T > G | Promoter | PCR-RFLP | 351 | 59˚C, 1.5 mM | MspAII | T allele-232, 88, 31 G allele-186, 88, 46, 31 | [29] |
| VEGFR1 | −710C/T | Promoter | PCR-RFLP | 665 | 65˚C, 1.5 mM | Nla III | C allele-665 T allele-520, 145 | [30] |