Standard strain

Recovered isolate

Phenotypic test

On B.A: small, gray and rough colonies with haemolysis appeared under Neath colonies.

On M.A: growth colonies appeared dark pink like pin point.

Staining : G−, cocobacili, single or pairs and clear bipolar with methylen blue

On B.A: small, gray and rough or smooth colonies with beta haemolysis around colony.

On M.A: lung isolate appeared as a pink pin point, while nasal isolate appeared as a pink mucoid

Staining: G-, cocobacili, single or pairs and clear bipolar with methylen blue.

Colony and staining characteristic

+

+

Indole test

_

Simmons citrate

_

_

SIM

yellow/yellow

yellow/yellow

TSI

Gelatinase test

+

+

Oxidase test

+

+

Catalase test

Urea test

+

+

Nitrate reduction test

+

+

Glucose acid

+

(except nasal isolate−) +

Lactose acid

weak +

Insitol acid

+

Sorbitol acid

+

(except nasal isolate+)−

Xylose acid

+

+

Rhamnose acid

+

Mannitol acid

+

Arabinose acid

+

Maltose acid