| Actual concentration | Within-run variations | Between-run variations | ||||
| Measured concentration | CV% | Accuracy (%) | Measured concentration | CV% | Accuracy (%) | |
| 625 ng/ml | 651.7 ± 51.5 | 7.87 | 104.2 ± 8.2 | 641.8 ± 46.1 | 7.23 | 102.8 ± 7.44 |
| 1250 ng/ml | 1286.7 ± 139.1 | 10.58 | 103.0 ± 10.9 | 1375 ± 85.2 | 6.16 | 110.1 ± 6.78 |
| 2500 ng/ml | 2596.6 ± 179.2 | 6.84 | 103.8 ± 7.1 | 2588 ± 195.7 | 7.54 | 103.4 ± 7.8 |
| 5000 ng/ml | 5078.3 ± 563.2 | 10.94 | 101.4 ± 11.1 | 5230 ± 562.9 | 10.75 | 104.5 ± 11.24 |