Parameter | Imp-1 | Imp-2 | Imp-3 | DRF |
System suitabilitya |
|
|
|
|
RT (min) | 4.18 | 20.26 | 17.98 | 9.78 |
RRT | 0.427 | 2.071 | 1.838 | 1.000 |
Rs | - | 3.47 | 13.92 | 16.14 |
k′ | - | 3.8 | 3.3 | 1.3 |
As | 1.22 | 0.98 | 1.06 | 1.02 |
N | 6935 | 16830 | 11003 | 6272 |
Linearityb |
|
|
|
|
Range (µg/mL) | 0.6-3.0 | 0.6-3.0 | 0.6-3.0 | 50-150 |
r2 | 0.9980 | 0.9992 | 0.9988 | 1.0000 |
Slope | 170.73 | 204.9 | 178.24 | 18808 |
Intercept | 292.1 | 194.2 | 339.2 | 27997 |
Sensitivityb |
|
|
|
|
LOD (µg/mL) | 0.07 | 0.20 | 0.20 | 0.15 |
LOQ (µg/mL) | 0.20 | 0.55 | 0.60 | 0.45 |
Precision (%RSD)a |
|
|
|
|
System | 1.27 | 0.32 | 1.20 | 0.23 |
Method | 2.56 | 1.41 | 1.77 | 0.79 |
Intermediate | 2.67 | 3.08 | 0.66 | 0.74 |
LOQ | 3.21 | 4.82 | 1.75 | - |
% Recovery (accuracy)b |
|
|
|
|
50% level | 98.8 | 95.6 | 98.9 | 98.7 |
100% level | 100.1 | 99.7 | 98.6 | 100.1 |
150% level | 97.4 | 96.0 | 99.6 | 100.6 |