Fragment

Forward primer (5' → 3')

Tm (˚C)

Reverse primer (5' → 3')

Tm (˚C)

Exon 2-1a

CTGAAGACCAAGAGGGAAGA

52

CAATGACGGACAGGAGAAAG

52

Exon 2-2a

CAAGGAGCAGAACGGAGTG

53

GGAGGCTGAGATGGGACTT

53

Exon 3

TCCGAGGCCCCAAACTAAA

51

ATGATGCGGCTGGCTTGCT

53

Exon 4-1a

TGATGGTGGCTCTGTGCTC

53

GTGGTCCCAAAAGTGTCGT

51

Exon 4-2a

GGGCGCTGCACTATCTCTT

53

TCCAACCAAGGGGCTACCA

53

Exon 5

TTGACAGCCACCCACAGAGT

54

AGCACAAAACCCAACTGAGG

52

Exon 6

GCGTCCCAGGAAATGTTTG

51

CCCTGTGGACTGTGAAGCA

53

Exon 7

GCATCTTCCACCAGTCGTCT

54

TGTTCTCATCCAGGGACACC

54

Exon 8

CCTTCCCCAGACACAGTAA

51

AAAAAGGCTTTGCTGAGAG

47

Exon 9

TTCAGCAGAGCCGCACCAG

55

GAACCCAACTGCCGAGGAC

55

Exon 10

CCGACCCTGTGCTCTGTGT

55

GTGCTGCGGTTCTGTCTGG

55

Exon 11

GGGTTGAATTTTAGGGACTC

50

CACAGCCACCAAACAAGAGG

54

Exon 12

ACTGATGCCACCTCTTCTCC

54

CTCCAGCCACAGTGACAACC

56

Exon 13

GCCTGACCTCCGTATCTGCT

56

ACACCCACGGAGCCTTTCTG

56

Exon 14

GTGAGGGTGCTGGTAGGTGA

56

CTGGGGGCTAAGAACACTGA

54