| Parameters/Bulls | Red Chittagong Cattle | BLRI Cattle Breed 1 | Munshiganj Cattle | Sig. level | |
| VAP (µm/s) | Fresh | 143.99 ± 4.15 | 156.68 ± 3.08 | 145.15 ± 27.10 | NS |
| Post-thaw | 107.25 ± 8.85 | 96.04 ± 0.78 | 121.82 ± 20.09 | NS | |
| VSL (µm/s) | Fresh | 128.50 ± 5.53 | 139.18 ± 3.83 | 137.13 ± 28.12 | NS |
| Post-thaw | 90.46 ± 7.12 | 69.00 ± 1.48 | 107.42 ± 22.32 | NS | |
| VCL (µm/s) | Fresh | 232.21 ± 23.59 | 256.06 ± 3.41 | 208.28 ± 54.84 | NS |
| Post-thaw | 188.62 ± 12.97 | 179.8 ± 1.41 | 186.92 ± 27.91 | NS | |
| STR (%) | Fresh | 88.35 ± 3.45 | 89.07 ± 0.38 | 93.73 ± 1.33 | NS |
| Post-thaw | 84.19 ± 2.33 | 73.21 ± 1.91 | 84.66 ± 5.63 | NS | |
| LIN (%) | Fresh | 58.57 ± 6.63 | 56.20 ± 2.13 | 69.46 ± 6.73 | NS |
| Post-thaw | 50.37 ± 1.59 | 37.79 ± 1.00 | 54.70 ± 5.75 | NS | |
| ALH (µm) | Fresh | 8.33 ± 1.62 | 9.53 ± 0.78 | 6.83 ± 1.97 | NS |
| Post-thaw | 8.84 ± 0.63 | 10.05 ± 0.80 | 8.10 ± 0.52 | NS | |
| BCF (Hz) | Fresh | 34.66 ± 4.97 | 32.96 ± 2.52 | 35.89 ± 3.36 | NS |
| Post-thaw | 25.77 ± 1.73ab | 20.19 ± 0.82b | 32.92 ± 3.42a | ** | |