Time (min) | Solvent Α (% v/v) | Solvent Β (% v/v) | Flow (ml/min) |
0 | 100 | 0 | 1 |
30 | 85 | 15 | 1 |
40 | 78 | 22 | 1 |
50 | 0 | 100 | 1 |
65 | 0 | 100 | 1 |
70 | 100 | 0 | 1 |