Purification step

Volume

Total activity (U/ml)

Total protein (mg/ml)

Specific gravity (µ/mg/protein)

Yield (%)

Purification

Supernatant

180

612

72

8.5

100

1

4M Sucrose

60

510

30

17

83

2

Q-Sepharose

45

460

10.8

42.5

75

5

Phenyl-Sepharose

15

219

3.3

66.4

35.8

7.8